Table 1 |
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|
Developmental expression of surf genes as seen by RT-PCR using primer set 1. surf genes exhibit a differential expression pattern during the different stages of the parasite cycle. |
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| surf Gene |
Time Post Invasion (hrs) |
SURFIN Grouping§ |
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|
|
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| PlasmoDB ID |
10 |
20 |
30 |
40 |
||
|
|
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| surf1.1 |
PFA0625w |
- |
- |
+ |
+ |
A |
| surf1.2 |
PFA0650w |
- |
- |
- |
- |
Truncated |
| surf1.3 |
PFA0725w |
+ |
+ |
+ |
+ |
B |
| surf4.1 |
PFD0105c, PFD0100c |
- |
- |
+ |
+ |
A |
| surf4.2 |
PFD1160w |
+ |
+ |
+ |
+ |
A |
| surf8.1 |
MAL8P1.1 |
- |
- |
- |
- |
B |
| surf8.2 |
PF08_0002 |
- |
- |
- |
- |
A |
| surf8.3 |
MAL8P1.162 |
+ |
+ |
+ |
+ |
B |
| surf13.1 |
PF13_0074, PF13_0075 |
- |
- |
- |
- |
Truncated |
| surf14.1 |
PF14_0747 |
- |
- |
+ |
- |
B |
|
|
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|
The genes that are expressed are indicated by "+" while those that are not expressed are indicated by "-". surf4.1 is expressed in the late stages of the cycle from ≈30 hrs all the way until the dividing merozoites. Three surf genes (surf1.3, surf4.2 and surf8.3) are expressed throughout the life cycle while others are expressed at a particular stage in the life cycle. + = Gene amplified. - = Gene not amplified. §SURFIN grouping is based on the number of exons [20] as well as protein alignments which have resulted in surf4.1 to be in group A even though it has 3 exons. The transmembrane domain in the group A paralogs is present in the first exon and in the second exon in group B paralogs |
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|
Mphande et al. Malaria Journal 2008 7:116 doi:10.1186/1475-2875-7-116 |
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