Table 2 |
||
|
Comparison of fluorescent dyes for dual staining of pRBCs in combination with Giemsa staining. |
||
|
Fluorescent dye |
Detection filter |
Giemsa and fluorescent dye (light/fluorescence)* |
|
|
||
|
SYBR Green 1 |
fluorescein |
+/+ |
|
Ethidium monoazide (EMA) |
rhodamine |
|
|
non photoactivated |
+/- |
|
|
photoactivated |
+/- |
|
|
Yoyo-1 |
fluorescein |
+/+ |
|
Acridine Orange |
rhodamine |
+/- |
|
Ethidium homodimer-2 [EthD-2]) |
" |
+/+ |
|
SYTO 44 |
fluorescein |
+/- |
|
SYTO 45 |
" |
+/- |
|
SYTO 17 |
rhodamine |
+/- |
|
SYTO 59 |
" |
+/- |
|
SYTO 61 |
" |
+/- |
|
SYTO 64 |
" |
+/- |
|
GelRed |
" |
+/- |
|
Hoescht 33258 |
DAPI |
+/- |
|
DAPI |
" |
+/- |
|
|
||
|
*Plus/minus detection of Giemsa or fluorescence |
||
|
Guy et al. Malaria Journal 2007 6:89 doi:10.1186/1475-2875-6-89 |
||
