Figure 4.

LEDs can be used for detecting SYBR stained pRBCs. Fluorescence emission from pRBCs excited with a 5 mW Blue LED powered by 4 AA batteries. The pRBCs were stained with SYBR Green 1 and were then examined using a Zeiss Axiostar Plus microscope with an LED light source fitted in place of its mercury lamp. Photographs depict fluorescent (left) and white light (right) images of a mature parasite (upper) and a ring stage parasite (lower). Fluorescent intensity of the stained parasites was comparable to that observed with the HBO 50/AC high intensity light source supplied by the manufacturer.