Table 1 |
||||
|
Effect of plasma samples, cytokines and parasite products in the white blood cell aggregation assay |
||||
| Expt. No. |
Sample |
% RCAI (Median) |
25% |
75% |
|
|
||||
| 1 |
NHP |
5.80 |
1.6 |
7.3 |
| 2 |
PP |
100 |
100.0 |
100.0 |
| 3 |
POPP |
20.90 |
5.6 |
28.4 |
| 4 |
NHP+cytokines |
17.20 |
6.7 |
23.5 |
| 5 |
NHP+PvAg |
18.60 |
4.5 |
20.8 |
| 6 |
NHP+PvAg+ cytokines |
88.50 |
79.8 |
92.4 |
| 7 |
NHP+RBC Ag+ cytokines |
10.00 |
2.4 |
12.9 |
| 8 |
POPP+cytokines |
18.30 |
7.5 |
22.3 |
| 9 |
POPP+PvAg |
16.40 |
9.4 |
18.1 |
| 10 |
POPP+PvAg+ cytokines |
77.10 |
64.7 |
79.8 |
| 11 |
NHP+LPS |
6.10 |
1.9 |
7.2 |
| 12 |
NHP+LPS+cytokines |
16.10 |
6.9 |
18.6 |
| 13 |
PP + IRS-Pv |
24.90 |
15.6 |
28.4 |
| 14 |
PP + IRS-Pf |
54.40 |
48.6 |
65.4 |
| 15 |
PP + CHS |
17.00 |
14.2 |
19.5 |
|
|
||||
|
% RCAI: % Relative Cell Aggregation Index 25%–75%: 25th and 75th percentiles Normal human plasmas (NHP) and post paroxysm plasmas (POPP) were reconstituted with freeze-thawed extracts of P. vivax schizonts (Pv Ag), normal red cell extracts (RBC Ag), recombinant human cytokines TNF-∝, GM-CSF, IL-6 and IL-10 (cytokines) or E. coli lipopolysaccharide (LPS). P. vivax paroxysm plasma (PP) was pre-incubated with rabbit serum raised against P. vivax schizonts (IRS-Pv), with rabbit serum raised against P. falciparum schizonts (IRS-Pf) or with convalescent human serum (CHS) collected 4 – 6 weeks after drug cure of a P. vivax infection, from semi-immune individuals resident in a malaria-endemic area prior to being tested in the cell aggregation assay. The table provides the median values together with the interquartile range (25%–75%). |
||||
|
Karunaweera et al. Malaria Journal 2007 6:62 doi:10.1186/1475-2875-6-62 |
||||
