Table 1

Mobile phase compositions for the separation of preservatives by TLC (detected at 254 nm)


Retardation factor (RF)

Mobile phase (v/v/v)
Methylparaben
Propylparaben
Sorbic acid
Benzoic acid

CH3OH/H2O (80/20)
0.70
0.57
0.68
0.69 (Faint spot)
CH3OH/H2O/CH3COOH (70/30/1)
0.47
0.28
0.47
0.50 (Faint spot)
CH3OH/H2O/CH3COOH (80/20/1)
0.75
0.67
0.75
0.75
CH3OH/H2O/CH3COOH (80/20/3)
0.57
0.48
0.64
0.64 (Faint spot)
CH3OH/H2O/NH3 (80/20/1)
0.80
0.70
0.87
Highly faint spot
CH3CN/H2O/CH3COOH (95/5/1)
0.88
0.81
0.84
Highly faint spot

Atemnkeng et al. Malaria Journal 2007 6:12   doi:10.1186/1475-2875-6-12

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