Comparison of PCR-based detection of Plasmodium falciparum infections based on single and multicopy genes

doi:10.1186/1475-2875-6-112

Malaria Journal

Volume 6

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Monday GC
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Kun JF

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Comparison of PCR-based detection of Plasmodium falciparum infections based on single and multicopy genes

Segun I Oyedeji¹^,2 , Henrietta O Awobode² , Gamaliel C Monday³ , Eric Kendjo¹^,4 , Peter G Kremsner¹^,4 and Jürgen F Kun¹

¹Institute for Tropical Medicine, Department of Parasitology, University of Tübingen Wilhelmstrasse 27, D-72074, Tübingen, Germany

²Parasitology Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria, Africa

³Clinical Service Unit, Nasarawa State Ministry of Health, Lafia, Nigeria, Africa

⁴Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon, Africa

author email corresponding author email

Malaria Journal 2007, 6:112doi:10.1186/1475-2875-6-112


Published:	16 August 2007

Abstract

PCR-based assays are the most sensitive and specific methods to detect malaria parasites.

This study compared the diagnostic accuracy of three PCR-based assays that do not only differ in their sequence target, but also in the number of copies of their target region, for the detection of Plasmodium falciparum in 401 individuals living in a malaria-endemic area in Nigeria. Compared to a composite reference generated from results of all the 3 PCR assays, the stevor gene amplification had a sensitivity of 100% (Kappa = 1; 95% CI = 1.000–1.000), 83% (Kappa = 0.718; 95% CI = 0.648–0.788) by SSUrRNA gene PCR and 71% (Kappa = 0.552; 95% CI = 0.478–0.627) by the msa-2 gene amplification.

Results from this study indicate that the stevor gene amplification is the most sensitive technique for the detection of P. falciparum. This assay may be an important reference standard, especially when a confirmatory technique with high sensitivity and specificity is needed for ruling out P. falciparum infection.