Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods
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* Corresponding author: Chris Bass chris.bass@bbsrc.ac.uk
- † Equal contributors
Malaria Journal 2007, 6:111 doi:10.1186/1475-2875-6-111
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BioMed Central: 5 citations
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Research
Christopher M Jones, Antoine Sanou, Wamdaogo M Guelbeogo, N'Fale Sagnon, Paul C D Johnson, Hilary Ranson Malaria Journal 2012, 11:24 (23 January 2012) The main finding here is that older mosquitoes are more susceptible to insecticides. This has important implications for understanding the epidemiological impact of resistance in field-based vector control interventions.
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Research
Extensive permethrin and DDT resistance in Anopheles arabiensis from eastern and central Sudan Yousif E Himeidan, Muzamil M Abdel Hamid, Christopher M Jones, Hilary Ranson Parasites & Vectors 2011, 4:154 (3 August 2011) High level of resistance to permethrin and DDT is reported in the malaria vector An. arabiensis from Sudan. The 1014F kdr allele is present in high frequency and associated with resistance to permethrin in six populations from eastern and central Sudan. Image: The WHO test tubes used for insecticides bioassays.
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Methodology
PCR-based methods for the detection of L1014 kdr mutation in Anopheles culicifacies sensu lato Om P Singh, Prerna Bali, Janet Hemingway, Sarala K Subbarao, Aditya P Dash, Tridibes Adak Malaria Journal 2009, 8:154 (14 July 2009) |
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Research
Frances C Ridl, Chris Bass, Miguel Torrez, Dayanandan Govender, Varsha Ramdeen, Lee Yellot, Amado Edu, Christopher Schwabe, Peter Mohloai, Rajendra Maharaj, Immo Kleinschmidt Malaria Journal 2008, 7:194 (29 September 2008) The paper addresses three aspects of malaria vectors in Equatorial Guinea: their abundance including species composition, their role in malaria transmission and finally incidence of insecticide resistance alleles. The demonstration of a high frequency of kdr alleles in mosquito populations before the onset of a malaria control programme shows the importance of continuous entomological surveillance.
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Methodology
Chris Bass, Dimitra Nikou, Andrew M Blagborough, John Vontas, Robert E Sinden, Martin S Williamson, Linda M Field Malaria Journal 2008, 7:177 (15 September 2008) In tests of PCR methods for the detection of different malaria parasites, the TaqMan method showed sensitivity and specificity at least as good as the gold standard nested PCR approach and, unlike the latter, was not inhibited by the storage of mosquito specimens by drying or in ethanol/isopropanol. The new method is also simple and rapid to perform and cheaper to run.
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