|
Primer pairs and restriction enzymes used for the identification of each pfcytb codon 268 polymorphism. |
||||||
| Codon 268 |
Type |
Primer pair |
Product size (bp) |
Restriction enzyme |
Restriction pattern |
Fragment length (bp) |
|
|
||||||
| Outer PCR |
OD ; OR |
716 |
||||
|
|
||||||
| TAT |
Wild type |
OD ; WtR |
600 |
Mph1103-I |
Cuts Wt, T802A |
570 (+30) |
| AAT |
T802A |
802D; 802R |
146 |
Dra-I |
Cuts T802A |
104 (+42) |
| TCT |
A803C |
803D; OR |
173 |
Cai-I |
Cuts A803C |
148 (+25) |
|
Wt: wild type; bp: base pairs; D: direct primer; R: reverse primer. | ||||||
Pimentel et al. Malaria Journal 2006 5:30 doi:10.1186/1475-2875-5-30 |
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