Table 1 |
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Primer sequences used for the amplification of pfcytb codon 268. |
|
| Primer |
Sequence |
|
|
|
| OD |
5'-CGCAACAGGTGCTTCTCTTGT-3' |
| OR |
5'-ACAGAATAATCTCTAGCACCAAAAATCAT-3' |
| WtR |
5'-GGTTTACTTGGAACAGTTTTTAACAaTG-3' |
| 802D |
5'-GTTTATTTGGAATTATACCTTTATCACATCCTGATAATGCTATC-3' |
| 802R |
5'-TAAACCAGCTGGTTTACTTGGAACAGTTTTTAACATTGtt-3' |
| 803D |
5'-CCTGAATGGTACTTTCTACCAgTT-3' |
|
|
|
|
Small case indicates base changes to provide digest sites for restriction enzymes to distinguish wild type from mutations at codon 268. O: outer PCR; D: direct primer; R: reverse primer; Wt: wild type. |
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Pimentel et al. Malaria Journal 2006 5:30 doi:10.1186/1475-2875-5-30 |
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