Figure 1.

Restriction digests for detection of pfcytb codon 268 mutations on field samples. A. 600 bp amplification product with the primer pair for wild type (TAT) detection, digested by Mph1103-I; lane 1 and 7 – non-digested PCR product; lane 2 – K1 wild type; lanes 3–6, 8 field isolates. MWM – 100 bp molecular weight marker. B. 146 bp amplification product with the primer pair for mutation T802A (AAT) detection, digested by Dra-I; lane 1 and 7 – non-digested PCR product; lane 2 – K1 wild type; lanes 3–6 field isolates. MWM – 100 bp molecular weight marker. C.173 bp amplification product with the primer pair for mutation A803C (TCT) detection, digested by Cai-I; lane 1 and 7 – non-digested PCR product; lane 2 – K1 wild type; lanes 3–6 field isolates. MWM – 100 bp molecular weight marker.