Methodology
Using green fluorescent malaria parasites to screen for permissive vector mosquitoes
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* Corresponding author: Friedrich Frischknecht freddy.frischknecht@med.uni-heidelberg.de
1 Unité de Biologie et Génétique du Paludisme, Institut Pasteur, 25-28 Rue du Dr Roux, 75015 Paris, France
2 Centre de Production et d'Infection des Anopheles, Institut Pasteur, 25-28 Rue du Dr Roux, 75015 Paris, France
3 Department of Parasitology, University of Heidelberg Medical School, Tel 49-6221-566537, Fax 49-6221-564643, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
4 CNRS, Institut Alfred Fessard, Neurobiologie Génétique et Intégrative, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France
Malaria Journal 2006, 5:23 doi:10.1186/1475-2875-5-23
Published: 28 March 2006Additional files
Movie 1. Sporozoites in the haemolymph of the mosquito wing. Two sporozoites are stuck in a vein of an Anopheles stephensi wing. Other sporozoites pass by with the flow of the haemolymph. 5 frames per second, movie length: 8 seconds. For best viewing loop the movie. Size: 4.4 MB.
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Movie 2. Sporozoites in the hemolyph of an A. stephensi tibia. A large number of sporozoites float with the haemolymph in both directions. 1 frame per second, movie length: 74 seconds. Size: 3.3 MB.
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Movie 3. Sporozoites in the salivary gland of an Anopheles albimanus mosquito. The gland was carefully isolated from an infected mosquito and placed in RPMI containing 5% foetal calf serum in a glass-bottom well dish. Note the displacement of sporozoites, mainly in a typical back-and-forth fashion. 0.1 frames per second, movie length: 100 seconds. For best viewing loop the movie. Size: 2 MB.
Format: MOV Size: 2.1MB Download file
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