Table 1 |
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|
Characteristics of primers used in nested PCR on P. falciparum DHFR. Lower case denotes nucleotide mismatch. |
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| Reaction |
Primer |
Nucleotide location |
Product size (bp) |
|
|
|||
| Nest I |
U1: 5'-GGAAATAAAGGAGTATTACCATG-3' |
121..143 |
|
| U2: 5'-TAAGGTTCTAGACAATATAACA-3' |
393..372 |
273 |
|
| Nest II |
U3: 5'-GAAATGTAaTTCCCTAGATATGgAATATT-3' |
144..172 |
|
| U4: 5'-ATTTATCCTATTGCTTAAAGGT-3' |
372..351 |
229 |
|
| Nest I |
M1: 5'-TTTATGATGGAACAAGTCTGC-3' |
3..18 |
|
| M5: 5'-AGTATATACATCGCTAACAGA-3' |
645..625 |
643 |
|
| Nest II |
F: 5'-GAAATGTAATTCCCTAGATATGgAATATT-3' |
144..172 |
|
| M4: 5'-TTAATTTCCCAAGTAAAACTATTAGAgCTTC-3' |
469..439 |
326 |
|
|
|
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|
Mharakurwa et al. Malaria Journal 2006 5:103 doi:10.1186/1475-2875-5-103 |
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