Figure 4.

GST-10 kDa/6His-PfJ solution binding assays. GST-10 kDa (200, 300, 400, 1000 and 2000 ng) bound to ~700 ng 6His-PfJ on His-Select™ magnetic beads in a concentration dependent manner (A-C). Protein complexes were resolved on 12% Laemmli gels and analyzed with scanning densitometry. Non-specific binding of heat denatured GST-10 kDa (54 ng/μg PfJ) was subtracted from the amount of bound protein. A, B and C represent independent experiments using two different protein preparations.