Table 1 |
||||||||
|
Primers used for genotyping P. vivax parasites. |
||||||||
| Gene |
Primer |
Sequencea |
mM Mg2+ b |
Annealing °C b |
Cyclesb |
|||
|
|
||||||||
| 1st |
2nd |
1st |
2nd |
1st |
2nd |
|||
|
|
||||||||
| Pvcs |
VCS-OF |
ATGTAGATCTGTCCAAGGCCATAAA |
1 |
58 |
25 |
|||
| VCS-OR |
TAATTGAATAATGCTAGGACTAACAATATG |
1 |
58 |
25 |
||||
| VCS-NF |
GCAGAACCAAAAAATCCACGTGAAAATAAG |
1 |
62 |
30 |
||||
| VCS-NR |
CCAACGGTAGCTCTAACTTTATCTAGGTAT |
1 |
62 |
30 |
||||
| Pvmsp1 F1 |
VM1-O1R |
CCACTCCATGAAACTGAAGTGTTTA |
2 |
68 |
25 |
|||
| VM1-N1F |
CGATATTGGAAAATTGGAGACCTTCATCAC |
2 |
2 |
68 |
68 |
25 |
30 |
|
| VM1-N1R |
CTTTTGCGCCTCCTCCAGCTGGCTCGTGT |
2 |
68 |
30 |
||||
| Pvmsp1 F2 |
VM1-O2F |
GATGGAAAGCAACCGAAGAAGGGAAT |
1 |
50 |
25 |
|||
| VM1-O2R |
AGCTTGTACTTTCCATAGTGGTCCAG |
1 |
1 |
50 |
64 |
25 |
35 |
|
| VM1-N2F |
AAAATCGAGAGCATGATCGCCACTGAGAAG |
1 |
64 |
35 |
||||
| Pvmsp1 F3 |
VM1-3F |
CAAGCCTACCAAGAATTGATCCCCAA |
1 |
66 |
42 |
|||
| VM1-3R |
ATTACTTTGTCGTAGTCCTCGGCGTAGTCC |
1 |
66 |
42 |
||||
|
|
||||||||
|
a Sequences are provided 5'- to 3'-end.p b The two columns indicate conditions for the primary and secondary amplification reactions. |
||||||||
|
Imwong et al. Malaria Journal 2005 4:20 doi:10.1186/1475-2875-4-20 |
||||||||
