Table 2

Double blind trial of HOLA approach versus conventional PCR

Specimen

HOLA

PCR 1^e

PCR 2^e

NK5^a

SS

SS

SS

NK6

SS

X

SS

NK7

RR

RR

RR

NK8

SS

SS

SS

Kenya 1^b

SS

X

SS

Kenya 2

SS

X

SS

Kenya 3

RS

X

RR

Thyolo 7^c

SS

SS

SS

Thyolo 33

SS

SS

SS

Thyolo 34

SS

X

SS

Thyolo 64

SS

X

SS

Thyolo 75

SS

X

SS

RSP^d

RR

RR

RR

^aSpecimens labelled NK collected by Pie Muller, Ben Oloo, and Nadine Randle from Asembo, Kenya on 05/2004, DNA extracted by Ballinger-Crabtree method [15] on 09/2004. ^bSpecimens labelled Kenya collected by Pie Muller, Ben Oloo, and Nadine Randle from Asembo Bay, Kenya on 05/2004 DNA extracted by Livak method [14] on 08/2004. ^cSpecimens labelled Thyolo collected by Philimon Tambala and Bill Hawley from Thyolo, Malawi on 01/1995, DNA extracted by Ballinger-Crabtree method [15] on 09/1997. ^dSpecimen from RSP colony. Abbreviations: SS, homozygous susceptible. RR, homozygous resistant. RS, heterozygous. ^e Conditions for the PCR reactions were identical.

Lynd et al. Malaria Journal 2005 4:16   doi:10.1186/1475-2875-4-16