Figure 3.

Correlation of expression levels derived from the oligonucleotide array with ng cDNA determined by real time PCR. Log ng of cDNA from the Nigerian sample and the in vitro 3D7 ring stage sample was determined for five genes that vary from high to low level abundance by array: PFB0120, PFB0100, PFB0270, PFB0355, and PFB0065. cDNA concentration was determined with real time PCR using a standard curve based on 3D7 genomic DNA. These results were correlated to the expression units (EU) found by the array. (R² = correlation coefficient)