Table 2

V_Hand V_Lassignments and alignment of CDR 3 sequences The selected (NP 04, 12, 13 designated Pf NPNA-1 bind to the repeat epitope), all other NP clones were randomly picked after the panning procedure and were subsequently shown not to be reactive with the repeat epitope. Non-selected (R01-10) were randomly picked from the library prior to initiating panning. The peptide sequence of the heavy and light chain complementarity-determining region 3 (CDR3) is shown below. V_H/V_Lfamilies, segments and the number of differences from germline segments were determined by using the V BASE sequence directory (Tomlinson, I. M., Williams, S. C., Corbett, S. J., Cox, J. P. L. & Winter, G., MRC Centre for Protein Engineering, Cambridge, UK) and the DNAPLOT alignment package (Müller, W. & Althaus, H.-H., Köln University)
clone code*	V_Hfamily	V_HSegment	Differences from germline	V_HCDR3	V_Lfamily	V_LSegment	Differences from germline	V_LCDR3

PfNPNAl	VH3	DP46	10	DRDSSSYFDS	VkI	L12a	15	QQYNSYSGLT
NP04, NP12, NP13	VH3	DP46	10	DRDSSSYFDS	VkI	L12a		QQYNSYSGLT
R01	VH1	4M28^†‡	28(+6)* ^§-	DSESVAQWRY	VkIV	DPK24	43	QQSLSPVWT
R02	VH3	COS-3^‡	27 (+3)_	GVNWCSDY	VkI	DPK9	10	QQSYSTSWT
R03	VH5	DP73	35	LYTSIYYFDS	VkIV	DPK24	7	QQYYSTPLT
R04	VH3	DP46	8	DRVTNFWSGYFDY	VkIII	DPK22	13	QQYGSSPGFT
R05	VH3	DP58	23	DSTVKTVTKMRYGLD V	VkIII	DPK22	8	QQYGSSPFT
R06	VH1	4M28^†	12	DNYGDPGGGFDI	VkIII	DPK22	11	QQYGNSPRT
R07	VH5	DP73	9	RFWFGELYDAFDI	VkIV	DPK24	16	HQYYSTPQT
R08	VH5	DP73	34	LYTSIYYFDS	VkIII	DPK22	14	QQYGRSPWT
R09	VH3	V3-21^†	34	DQGGGWSSEVDS	VkIII	Vg	5	QQRSNWPLT
R10	VH1	DP7^‡	21 (+9)**	ALYGHDAFDI	VkI	DPK4	12	PKYNSALHT
NP02	VH3	DP47	36	ERPYDAFDS	VkIII	DPK22	23	QQYSTSPPMYN
NP03	VH5	DP73	40	LYTSIYYFDS	VkIII	Vg	17	KQRSKWPPIT
NP05	VH3	V3-48	14	EPRGAGTTLYFDY	VkIII	DPK22	22	QQYGGSPGYN
NP08	VH4	4.30^†	18	DRGVSSGWTFDC	VkII	DPK16	32	MQLTAFPWT
NP09	VH4	DP71	17	FRGGVAAGYDY	VkIII	DPK22	24	QHYRESCS
NP10	VH4	DP78	29	DRVRVPYYYIDV	VkIII	DPK22	15	QQYGTSPYS
NP11	VH3	VH3-8^†	12	DTTVTHYFDY	VkI	DPK9	21	QQSFSSPRT
NP14	VH1	DP88	20	GPGATIHYYYMDV	VkI	DPK8	18	QQLDNYPLT
NP15	VH5	DP73	36	LYTSIYYFDS	VkIII	DPK22	28	QQYGNSPPT

Phage clones were either selected from the library at random (prefix R) or after four rounds of panning against (NPNA)₃C-BSA, eluting with free (NPNA)₃peptide (prefix NP). ^†The segment given the best DNAPLOT match, although the segment sequence has not been verified by duplication. ^‡Aligned after removal of the unusual sequence additions (see^§,-, ^‡,-, ). ^§Figure in brackets indicates an unusual sequence addition. - Sequence has two additional codons in CDR1. - Sequence has one additional codon in CDR1. * Sequence has three additional codons in CDR2.
Chappel et al. Malaria Journal 2004 3:28 doi:10.1186/1475-2875-3-28