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Method for detecting codon 268 mutations: primer pairs for amplification with size of amplification product and restriction enzyme for following digest |
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| Codon 268 |
Type |
Primer pair |
Product size |
Cutting enzyme |
Fragment length |
|
|
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| Outer PCR |
CYTb1 + CYTb2 |
939 bp |
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| TAT |
Wild type |
CYTb3 + CYTb5 |
384 bp |
NsiI |
359 + 25 |
| TCT |
Y268S |
CYTb2 + CYTb6 |
171 bp |
AlwNI |
147 + 24 |
| AAT |
Y268N |
CYTb2 + CYTb7 |
174 bp |
SspI |
150 + 24 |
|
NsiI cuts TAT; AlwNI cuts TCT; SspI cuts TAT and TCT, but not AAT | |||||
Schwöbel et al. Malaria Journal 2003 2:5 doi:10.1186/1475-2875-2-5 |
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