Table 1

Method for detecting codon 268 mutations: primer sequences for outer and nested PCR (5' -> 3')

Primer Name
Sequence

CYTb1
CTCTATTAATTTAGTTAAAGCACA
CYTb2
ACAGAATAATCTCTAGCACC
CYTb3
AGCAGTAATTTGGATATGTGGAGG
CYTb5
GGTTTACTTGGAACAGTTTTTAACAaTG *
CYTb6
TGAATGGTACTTTCTACCAgTT *
CYTb7
ACCTGAATGGTACTTTCTACaATaT *

* Small letters indicate base changes to provide digest sites for restriction enzymes to distinguish wild type from mutations at codon 268

Schwöbel et al. Malaria Journal 2003 2:5   doi:10.1186/1475-2875-2-5