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Detection of Plasmodium falciparum and Plasmodium vivax subclinical infection in non-endemic region: implications for blood transfusion and malaria epidemiology

Luciana MF Maselli, Debora Levy, Gabriel Z Laporta, Aline M Monteiro, Linah A Fukuya, Maria F Ferreira-da-Cruz, Claudio T Daniel-Ribeiro, Pedro E Dorlhiac-Llacer, Maria Anice M Sallum and Sérgio P Bydlowski*

  • * Corresponding author: Sérgio P Bydlowski

  • † Equal contributors

Malaria Journal 2014, 13:224  doi:10.1186/1475-2875-13-224

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Sergio Bydlowski   (2014-07-05 08:36)  University of Sao Paulo School of Medicine

We have unfortunately found some mistakes in our recently published manuscript.

 Second paragraph, lines 19-23

 “…however, in 2013, 146 autochthonous cases of malaria were identified in São Paulo State, representing 22% of the total occurring outside the Amazon region. Moreover, there was a 256% increase compared to 2006 [12].”

should be replaced by

 “…however, from 1985 to 2006, 83 autochthonous cases of malaria were identified on the northern coast of São Paulo State [12], and from 2000 to 2013, 320 autochthonous cases were registered in the São Paulo State (Brazilian National Malaria Control Programme 2014, Secretary for Health Surveillance, Ministry of Health, Brazil. The data can be obtained by request).”

 Section “Participants and samples”, 1st paragraph, lines 20-22.

 “…it was knew who would be next to donate blood nor who would be included in the study”

should be replaced by

 “…it was unknown who would be next to donate blood and who would be included in the study”.


Section “Plasmodium identification by real-time PCR”, 2nd paragraph, lines 11-13.

“…followed by 45 cycles of amplification (95°C for 15 s, 52°C for 60 s, and 72°C for 60 s).”

should be replaced by

 “…followed by 50 cycles of amplification (95°C for 15 s, and 60°C for 60 s).”


Reference 30

Gama BE, Silva-Pires Fdo E, Lopes MN, Cardoso MA, Britto C, Torres KL, de Mendonça Lima L, de Souza JM, Daniel-Ribeiro CT, Ferreira-da-Cruz M: Real-time PCR versus conventional PCR for malaria parasite detection in low-grade parasitemia. Exp Parasitol 2007, 116:427–432.”

Should be replaced by

Perandin F, Manca N, Calderaro A, Piccolo G, Galati L, Ricci L, MediciMC, Arcangeletti MC, Snounou G, Dettori G, Chezzi C: Development of a real-time PCR assay for detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for routine clinical diagnosis. J Clin Microbiol 2004, 42:1214–1219.


We are deeply sorry for these mistakes and for any inconvenience.

Competing interests



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