Open Access Research

Mitochondrial genome sequences reveal deep divergences among Anopheles punctulatus sibling species in Papua New Guinea

Kyle Logue123, Ernest R Chan1, Tenisha Phipps23, Scott T Small3, Lisa Reimer34, Cara Henry-Halldin3, Jetsumon Sattabongkot5, Peter M Siba4, Peter A Zimmerman23* and David Serre1*

Author Affiliations

1 Genomic Medicine Institute, Cleveland Clinic, 9500 Euclid Ave./NE50, 44195, Cleveland, OH, USA

2 Department of Biology, Case Western Reserve University, 44106, Cleveland, USA

3 Center for Global Health and Diseases, Case Western Reserve University, Biomedical Research Building, Room E426, 2109 Adelbert Road, 44106-4983, Cleveland, OH, USA

4 Papua New Guinea Institute of Medical Research, 441, Goroka, Papua New Guinea

5 Faculty of Tropical Medicine, Mahidol University, 10400, Bangkok, Thailand

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Malaria Journal 2013, 12:64  doi:10.1186/1475-2875-12-64

Published: 14 February 2013

Abstract

Background

Members of the Anopheles punctulatus group (AP group) are the primary vectors of human malaria in Papua New Guinea. The AP group includes 13 sibling species, most of them morphologically indistinguishable. Understanding why only certain species are able to transmit malaria requires a better comprehension of their evolutionary history. In particular, understanding relationships and divergence times among Anopheles species may enable assessing how malaria-related traits (e.g. blood feeding behaviours, vector competence) have evolved.

Methods

DNA sequences of 14 mitochondrial (mt) genomes from five AP sibling species and two species of the Anopheles dirus complex of Southeast Asia were sequenced. DNA sequences from all concatenated protein coding genes (10,770 bp) were then analysed using a Bayesian approach to reconstruct phylogenetic relationships and date the divergence of the AP sibling species.

Results

Phylogenetic reconstruction using the concatenated DNA sequence of all mitochondrial protein coding genes indicates that the ancestors of the AP group arrived in Papua New Guinea 25 to 54 million years ago and rapidly diverged to form the current sibling species.

Conclusion

Through evaluation of newly described mt genome sequences, this study has revealed a divergence among members of the AP group in Papua New Guinea that would significantly predate the arrival of humans in this region, 50 thousand years ago. The divergence observed among the mtDNA sequences studied here may have resulted from reproductive isolation during historical changes in sea-level through glacial minima and maxima. This leads to a hypothesis that the AP sibling species have evolved independently for potentially thousands of generations. This suggests that the evolution of many phenotypes, such as insecticide resistance will arise independently in each of the AP sibling species studied here.

Keywords:
Anopheles; Anopheles punctulatus sibling species; Molecular evolution; Molecular dating