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Open Access Highly Accessed Methodology

Evaluation of Plasmodium falciparum gametocyte detection in different patient material

Katharina Kast1, Nicole Berens-Riha12*, Ahmed Zeynudin4, Nuredin Abduselam4, Teferi Eshetu4, Thomas Löscher12, Andreas Wieser23, Jonathan Shock5 and Michael Pritsch12

Author Affiliations

1 Division of Infectious Diseases and Tropical Medicine, Medical Centre of the University of Munich (LMU), Leopoldstrasse 5, 80802 Munich, Germany

2 German Centre for Infection Research (DZIF), partner site Munich, Leopoldstrasse 5, 80802 München (Munich), Germany

3 Max von Pettenkofer-Institute of Hygiene and Medical Microbiology, Leopoldstrasse 5, 80802 Munich, Germany

4 Department of Microbiology, Parasitology and Immunology, Jimma University, Jimma, Ethiopia

5 The Laboratory for Quantum Gravity & Strings, Department of Mathematics and Applied Mathematics, University of Cape Town, Cape Town, South Africa

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Malaria Journal 2013, 12:438  doi:10.1186/1475-2875-12-438

Published: 4 December 2013

Abstract

Background

For future eradication strategies of malaria it is important to control the transmission of gametocytes from humans to the anopheline vector which causes the spread of the disease. Sensitive, non-invasive methods to detect gametocytes under field conditions can play a role in monitoring transmission potential.

Methods

Microscopically Plasmodium falciparum-positive patients from Jimma, Ethiopia donated finger-prick blood, venous blood, saliva, oral mucosa and urine samples that were spotted on filter paper or swabs. All samples were taken and stored under equal, standardized conditions. RNA was extracted from the filter paper and detected by real-time QT-NASBA. Pfs16-mRNA and Pfs25-mRNA were measured with a time to positivity to detect gametocyte specific mRNA in different gametocyte stages. They were compared to 18S-rRNA, which is expressed in all parasite stages. Results were quantified via a known dilution series of artificial RNA copies.

Results

Ninety-six samples of 16 uncomplicated malaria patients were investigated. 10 (66.7%) of the slides showed gametocyte densities between 0.3-2.9 gametocytes/μl. For all RNA-targets, molecular detection in blood samples was most sensitive; finger-prick sampling required significantly smaller amounts of blood than venous blood collection. Detection of asexual 18S-rRNA in saliva and urine showed sensitivities of 80 and 67%, respectively. Non-invasive methods to count gametocytes proved insensitive. Pfs16-mRNA was detectable in 20% of urine samples, sensitivities for other materials were lower. Pfs25-mRNA was not detectable in any sample.

Conclusions

The sensitivity of non-invasively collected material such as urine, saliva or mucosa seems unsuitable for the detection of gametocyte-specific mRNA. Sensitivity in asymptomatic carriers might be generally even lower. Finger-prick testing revealed the highest absolute count of RNA copies per μL, especially for Pfs25-mRNA copies. The method proved to be the most effective and should preferably be applied in future transmission control and eradication plans. A rapid test for gametocyte targets would simplify efforts.