Table 1

Sensitivity of different methods of parasite detection used in studies of mixed infection

Detection method

References

Species

Gold standard

Sensitivity

Specificity

Quoted lower limit


1

CS-ELISA

Wirtz et al.,1985 [17]

PV-VK210

PV-VK247

125-250 sporozoites per 30 μL mosquito extract


2

CS-ELISA

Wirtz et al.,1987 [18]

PF

100 sporozoites per mosquito


3

CS-ELISA

Collins et al.,1988 [19]

PM

25-50 sporozoites per mosquito


4

PCR on pfdhfr-ts

Harada et al.,2000 [20]

PF

4 pg of DNA (100 P/μL)


5

PCR on ssrRNA

Snounou et al.,1993 [21,22]

PF, PV, PM, PO

10 P/μL


6

Semi-nested PCR on ssrRNA

Larduex et al.,2008 [23]

PF, PV, PM, PO

3 sporozoites (0.06 pg of DNA)


Rubio et al.,1999 and 2002 [24,25]

Thick- thin films

100%

67%

0.1 P/μL


7

PCR-RFLP on Cyt B gene

Hasan et al,2009 [26]

PF, PV-VK210,

PV-VK247

0.2 pg of DNA


8

Real-time PCR ssrRNA

Mangold et al.,2005 [27]

PF, PV, PM, PO

Microscopy analysis

PF 94.1% PV100% PO 100%

PF100% PV99.1% PO100%

0.01-0.02% parasitaemia


9

RT-PCR on pfg377 and pfs16 mRNA

Nakazawa et al.,2009 [28]

PF

1 parasite per salivary gland


10

Real-time PCR 18S rRNA

Perandin et al.,2004 [29]

PF, PV, PO

Nested-PCR

100%

100%

PF 0.7P/μL, PV 4P/μL, PO 1.5P/μL


11

Dipstick

Bangs et al.,2002 [30]

PF, PV-VK210, PV-VK247

CS-ELISA

100%

97%

-


12

Vectest Dipstick

Ryan et al.,2002 [31]

PF, PV-VK210, PV-VK247

CS-ELISA

92%

98.10%

-


13

Fluorescein-labelled DNA probe

Lulu et al.,1997 [32]

PF

500 sporozoites


14

Microtiter plate hybridization

Kimura et al.,1997 [33]

Tangin et al.,2008 [34]

PF, PV, PM, PO

0.0001-0.0005% parasitaemia


P: malaria parasite, CS: circumsporozoite protein, pfdhfr-ts: Plasmodium falciparum dihydrofolate reductase-thymidylate synthase. PF: Plasmodium falciparum, PV: Plasmodium vivax, PO: Plasmodium ovale, PM: Plasmodium malariae.

Imwong et al. Malaria Journal 2011 10:253   doi:10.1186/1475-2875-10-253

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