Figure 5.

P. falciparum LSA-1 in human liver hepatocytes. P. falciparum sporozoites were injected intravenously into transgenic, chimeric mice possessing functioning human livers. Liver nodules were collected 5 or 6 days after injection, fixed and sectioned. Sections containing developing parasites were probed with antibody and detected by immunofluorescence. (A) A 5-day infected liver section probed with mouse polyclonal sera against LSA-NRC. (B) A 6-day infected liver treated as in (A). (C) A 6-day infected liver probed with mAb 71A3F1 that recognizes the TG2 formed isopeptide bond between glutamine and lysine. (D) As in (C) but using another mAb, 81D1C2, that also recognizes the TG2 isopeptide bond [52]